Product nameRabbit Anti-Human IgM H&L
See all IgM secondary antibodies
Tested applicationsSuitable for: ELISA, Dot blotmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound
Our Abpromise guarantee covers the use of ab8505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
ab8505 has not yet been referenced specifically in any publications.