Overview

  • Product name

    Rabbit IgA ELISA Kit
    See all IgA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum < 10%
    Inter-assay
    Sample n Mean SD CV%
    Serum < 10%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    ELISA set
  • Sensitivity

    5.137 ng/ml
  • Range

    6.25 ng/ml - 400 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 6.25ng/ml - 400ng/ml

  • Assay time

    0h 50m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rabbit
  • Product overview

    IgA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA protein in rabbit biological samples.

  • Notes

    In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP Conjugated Enzyme Antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentration 1 x 50ml
    Anti-Rabbit IgA ELISA Microplate 1 unit
    Chromogen Substrate Solution 1 x 12ml
    Rabbit IgA Calibrator (Lyophilized) 1 x 1ml
    Stop Solution 1 x 12ml
  • Research areas

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6
    • Ig alpha 1 chain C region
    • Ig alpha 2 chain C region
    • IGHA
    • IGHA1
    • IGHA2
    • IGHAC
    • Immunoglobulin heavy constant alpha 1
    • Immunoglobulin heavy constant alpha 2
    • Immunoglobulin heavy constant alpha 2 A2m marker
    • PIgR
    • Poly-Ig receptor
    • polymeric immunoglobulin receptor
    • polymeric immunoglobulin receptor Secretory component
    see all

Applications

Our Abpromise guarantee covers the use of ab205092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205092 Rabbit IgA ELISA Kit.

Protocols

References

This product has been referenced in:

  • Liu S  et al. The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. Oncotarget 8:66528-66539 (2017). ELISA ; Rabbit . Read more (PubMed: 29029533) »
See 1 Publication for this product

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