Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [Ax32] to Rabbit IgG - BSA and Azide free
- Suitable for: ELISA
Product nameAnti-Rabbit IgG antibody [Ax32] - BSA and Azide free
See all Rabbit IgG primary antibodies
DescriptionHuman monoclonal [Ax32] to Rabbit IgG - BSA and Azide free
Tested applicationsSuitable for: ELISAmore details
The details of the immunogen for this antibody are not available.
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab272685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 5 µg/ml.|
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
- Ig gamma chain C region antibody
- Ig kappa-b4 chain C region antibody
- Ig kappa-b5 chain C region antibody
Reverse ELISA - Anti-Rabbit IgG antibody [Ax32] - BSA and Azide free (ab272685).
Antigen: Biotinylated standard (rabbit IgG, rabbit IgM, bovine IgG) used at 0-0.01µg/ml with 2-fold dilutions carried out over 7 points.
Primary antibody range: 5µg/ml.
Secondary antibody is an HRP-Streptavidn used at 0.05µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272685 has not yet been referenced specifically in any publications.