Recombinant Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
![ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)](https://www.abcam.com/ps/products/190/ab190495/Images/ab190495-225832-anti-rabbit-igg-fab-region-antibody-rmg01-elisa.jpg "ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [RMG01] to Rabbit IgG Fab region
- Suitable for: WB, ELISA
- Reacts with: Rabbit
Overview
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Product name
Anti-Rabbit IgG Fab region antibody [RMG01] -
Description
Goat monoclonal [RMG01] to Rabbit IgG Fab region -
Host species
Goat -
Specificity
ab190495 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Rabbit
Does not react with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Rabbit IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab190495 purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG01 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.
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| ELISA |
Use a concentration of 0.01 - 0.5 µg/ml.
|
| Notes |
|---|
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa. |
|
ELISA
Use a concentration of 0.01 - 0.5 µg/ml. |
Images
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ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
ELISA analysis of different species’ IgG shows ab190495 reacted to the Fab region of Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG as the secondary antibody.
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![ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)](https://www.abcam.com/ps/products/190/ab190495/Images/ab190495-225831-anti-rabbit-igg-fab-region-antibody-rmg01-elisa.jpg)
ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)Titer ELISA analysis of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG was used as the secondary antibody.
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![Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)](https://www.abcam.com/ps/products/190/ab190495/Images/ab190495-225830-anti-rabbit-igg-fab-region-antibody-rmg01-western-blot.jpg)
Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)All lanes : Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495) at 0.2 µg/ml
Lane 1 : Non-reduced Rabbit IgG
Lane 2 : Reduced Rabbit IgG
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 150 kDa -
![Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)](https://www.abcam.com/ps/products/190/ab190495/Images/ab190495-4-benefits-of-recombinant-antibodies.png)
Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab190495 has been referenced in 3 publications.
- Ren B et al. Effect of BRAF-mediated PI3K/Akt/mTOR pathway on biological characteristics and chemosensitivity of NSCLC A549/DDP cells. Oncol Lett 22:584 (2021). PubMed: 34122635
- Lyu C et al. ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Exp Ther Med 12:4067-4071 (2016). PubMed: 28105136
- Li W et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). ChIP . PubMed: 25644173