Recombinant

Recombinant Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

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ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
  • ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
  • Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
  • Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Goat monoclonal [RMG01] to Rabbit IgG Fab region
  • Suitable for: WB, ELISA
  • Reacts with: Rabbit

Overview

  • Product name

    Anti-Rabbit IgG Fab region antibody [RMG01]

  • Description

    Goat monoclonal [RMG01] to Rabbit IgG Fab region

  • Host species

    Goat

  • Specificity

    ab190495 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Rabbit
    Does not react with: Mouse, Rat, Human

  • Immunogen

    Full length protein corresponding to Rabbit IgG.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab190495 purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RMG01

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.
ELISA
Use a concentration of 0.01 - 0.5 µg/ml.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.
ELISA
Use a concentration of 0.01 - 0.5 µg/ml.

Images

  • ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

    ELISA analysis of different species’ IgG shows ab190495 reacted to the Fab region of Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG as the secondary antibody.

  • ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    ELISA - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

    Titer ELISA analysis of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG was used as the secondary antibody.

  • Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    Western blot - Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    All lanes : Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495) at 0.2 µg/ml

    Lane 1 : Non-reduced Rabbit IgG
    Lane 2 : Reduced Rabbit IgG

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 150 kDa

  • Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
    Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)

References (2)

Publishing research using ab190495? Please let us know so that we can cite the reference in this datasheet.

ab190495 has been referenced in 2 publications.

  • Lyu C  et al. ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Exp Ther Med 12:4067-4071 (2016). PubMed: 28105136
  • Li W  et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). ChIP . PubMed: 25644173

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