Recombinant Anti-Rabbit IgG Fc antibody [RMG02] (ab190492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [RMG02] to Rabbit IgG Fc
- Suitable for: ELISA, WB
- Reacts with: Rabbit
Overview
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Product name
Anti-Rabbit IgG Fc antibody [RMG02] -
Description
Goat monoclonal [RMG02] to Rabbit IgG Fc -
Host species
Goat -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Rabbit -
Immunogen
Full length protein corresponding to Rabbit IgG Fc .
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Positive control
- WB: Non-reduced rabbit IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab190492 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG02 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 0.01 - 0.5 µg/ml.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.
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Notes |
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ELISA
Use a concentration of 0.01 - 0.5 µg/ml. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa. |
Target
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Database links
- Entrez Gene: 100009097 Rabbit
- SwissProt: P01870 Rabbit
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Alternative names
- Ig gamma chain C region antibody
- IGGC antibody
Images
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ELISA of IgGs from different species shows ab190492 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab190492 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab190492 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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All lanes : Anti-Rabbit IgG Fc antibody [RMG02] (ab190492) at 0.2 µg/ml
Lane 1 : Non-reduced rabbit IgG
Lane 2 : Reduced rabbit IgG
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 150 kDaab190492 reacs to non-reduced rabbit IgG.
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab190492 has been referenced in 25 publications.
- Wang Y et al. Overexpression of ARHI increases the sensitivity of cervical cancer cells to paclitaxel through inducing apoptosis and autophagy. Drug Dev Res 83:142-149 (2022). PubMed: 34189759
- Feng Z et al. Hydroxysafflor yellow A protects against ulcerative colitis via suppressing TLR4/NF-κB signaling pathway. Chem Biol Drug Des 99:897-907 (2022). PubMed: 35319164
- Feng J et al. PPARγ alleviates peritoneal fibrosis progression along with promoting GLUT1 expression and suppressing peritoneal mesothelial cell proliferation. Mol Cell Biochem 477:1959-1971 (2022). PubMed: 35380292
- Sun M et al. The A137R Protein of African Swine Fever Virus Inhibits Type I Interferon Production via the Autophagy-Mediated Lysosomal Degradation of TBK1. J Virol 96:e0195721 (2022). PubMed: 35412346
- Zhang Y et al. Runx1 promotes the development of glioma cells by regulating JAK-STAT signalling pathway. Arch Med Sci 18:761-776 (2022). PubMed: 35591830