Recombinant
RabMAb

Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (HRP) (ab199507)

Overview

  • Product name
    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (HRP)
    See all IgG isotype controls
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.

  • General notes

    KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR25A
  • Isotype
    IgG
  • Research areas
  • Cellular localization
    Secreted
  • Alternative names
    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Applications

Our Abpromise guarantee covers the use of ab199507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Images

  • IHC image of formalin-fixed paraffin-embedded normal mouse brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of formalin-fixed paraffin-embedded normal mouse spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of formalin-fixed paraffin-embedded normal rat brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of formalin-fixed paraffin-embedded normal rat spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of formalin-fixed paraffin-embedded normal human cerebral cortex sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • IHC image of formalin-fixed paraffin-embedded normal human spleen sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

    The background control image is taken from an identical assay without primary antibody or isotype control.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab199507 has not yet been referenced specifically in any publications.

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