Recombinant
RabMAb

Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free). Validated in IP, IHC, FuncS, Flow Cyt, ICC/IF. Cited in 5 publication(s).

Overview

  • Product name
    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)
  • Tested applications
    Suitable for: Functional Studies, IHC-P, Flow Cyt, ICC/IF, IPmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Endotoxin level: <1EU/mg (based on Lumulus Amebocyte lysate assay).
  • Clonality
    Monoclonal
  • Clone number
    EPR25A
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab199376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
IHC-P 1/250.
Flow Cyt 1/70.
ICC/IF 1/250.
IP Use at an assay dependent concentration.

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling KCTD5 with ab194825 at 1/70 dilution (red) compared with ab199376 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab199376 detects no signal in FC.

    Note: ab199376 detects no signal in Flow cytometry.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeled with ab199376 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing no staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab199376 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunohistochemistry analysis of rat colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on rat colon tissue was observed.

  • Immunohistochemistry analysis of mouse colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on mouse colon tissue was observed.

  • Immunohistochemistry analysis of human colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on human colon tissue was observed.

References

This product has been referenced in:
  • Theret L  et al. Identification of LRP-1 as an endocytosis and recycling receptor for ß1-integrin in thyroid cancer cells. Oncotarget 8:78614-78632 (2017). Read more (PubMed: 29108253) »
  • Guns LA  et al. Suramin increases cartilage proteoglycan accumulation in vitro and protects against joint damage triggered by papain injection in mouse knees in vivo. RMD Open 3:e000604 (2017). Read more (PubMed: 29299344) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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