Recombinant
RabMAb

Recombinant Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

Overview

  • Product name

    Rabbit IgG, monoclonal [SP137] - isotype control
    See all Rabbit isotype controls
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Chemical/ Small Molecule.

  • General notes

    Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP137
  • Isotype

    IgG
  • Research areas

  • Alternative names

    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab125938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling EPF with ab125938 at 1:100 dilution (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab125938) at 1:20 dilution (10.1 µg/ml)/ Black. Unlabeled control - Unlabelled cells / blue.
  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells showing staining with purified ab125938 at 1:20 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence analysis of Jurkat ( human T cell leukemia T lymphocyte) cells showing staining with purified ab125938 at 1:20 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

References

This product has been referenced in:

See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 13 Q&A

Question
Answer



We can recommend ab125938 (Rabbit IgG, monoclonal [SP137] - isotype control) as isotype control for rabbit monoclonal primary antibodies.

https://www.abcam.com/index.html?datasheet=125938 (or use the following: https://www.abcam.com/index.html?datasheet=125938).

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Answer

Thank you for contacting us.

Please note that the Isotype control should be used along with primary antibodies and the concentration of use should match the primary antibody.

The recommended 1/100 dilution is for guidelines only, customer have to optimize the conditions themselves. Regarding the antigen retrieval, it means, this can only be used with antibodies that requires antigen retrieval step.

The isotype control should not give you any staining so if it is showing high background then protocol optimization might be needed e.g. more diluted antibody or optimization of antigen retrieval solution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Unfortunately, in few cases we cannot find the connection between the antibody used in a publication and the antibody used in our catalog, especially as we are trying to link this kind of information to the datasheets.
However, as we have only two matching isotypes in this case, and both differ only in the amount we sell:
https://www.abcam.com/index.html?datasheet=125938 https://www.abcam.com/index.html?datasheet=125938.
https://www.abcam.com/index.html?datasheet=128142 https://www.abcam.com/index.html?datasheet=128142.

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Question
Answer



I've confirmed with the lab that there is no isotype subclass for rabbit IgG, unlike other species. So the isotype of ab125938 is just IgG.

Here is a reference that mentions the single rabbit isotype-

http://www.pnas.org/content/79/19/6018.full.pdf

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Answer

DISCOUNT CODE: ABTRIAL-TBSWY; ABTRIAL-TBSWC

Ablaufdatum: 2013-03-05

Wert: 370€; 360€


Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab92546 und ab125938 in Flow zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Die Discountcodes haben die Nummer ABTRIAL-TBSWY(ab92546) und ABTRIAL-TBSWC (ab125938), deckt den Wert von ab92546 bzw.ab125938 ab und können zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Flow eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurden weder ab92546 Anti-Cardiac Troponin T antibody [EPR3696] noch ab125938 Rabbit IgG, monoclonal [SP137] - isotype control bisher in Flow getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab92546 und ab125938 abdecken, und gegen eine erneute Bestellung eines weiteren Produktes von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab92546 und ab125938 in Flow testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie die Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie die Antikörper in Flow.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und wird dann bei der folgenden neuen Bestellung gegen den Preis von ab92546 und ab125938 verrechnet. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Answer

Thank you for your enquiry.

I can confirm that ab51074 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

The concentration of lot GR83749-1 is XXXmg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.

The concentration of the current lot of the rabbit monoclonal isotype control we discussed, ab125938, is 0.527 mg/ml. The concentration of the Nrf2 antibody is 0.2 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

My name is Jeremy Kasanov and Tania passed your enquiry to me. She mentions you are looking for a control for Mouse IgG2b and Rabbit Igg1. I am happy to help you find isotype controls for these antibodies. I just need to know:

1.Are your IgG2b and IgG1 antibodies unconjugated, and if sowhat they are conjugated to?

2. What is the concentration and storage buffer of the 2 primary antibodies.

Thanks in advance for the additional information.

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1-10 of 13 Q&A

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