Rabbit IgG, polyclonal - Isotype Control (ab37415)
- Datasheet
- References (66)
- Protocols
Overview
-
Product nameRabbit IgG, polyclonal - Isotype Control
See all Rabbit isotype controls -
SpecificityThis antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
-
Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details
-
General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 8.20
Constituents: 0.6% Boric Acid, 0.95% Sodium borate, 0.4% Sodium chloride -
Concentration information loading... -
PurityAffinity purified
-
Purification notesProtein A-purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
-
Alternative names
- rabbit isotype control
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab37415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 24961933 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 23995768 | |
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. | |
| ChIP/Chip | Use at an assay dependent concentration. PubMed: 20673990 |
Images
-
Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024.Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.
PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.
-
ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566.ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.
References
This product has been referenced in:
- Peng C et al. BCL6-Mediated Silencing of PD-1 Ligands in Germinal Center B Cells Maintains Follicular T Cell Population. J Immunol 202:704-713 (2019). Read more (PubMed: 30567732) »
- Cohrs G et al. Expression profiles of pro-inflammatory and pro-apoptotic mediators in secondary tethered cord syndrome after myelomeningocele repair surgery. Childs Nerv Syst 35:315-328 (2019). Read more (PubMed: 30280214) »
