Rabbit IgG, polyclonal - Isotype Control (ab37415)

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Overview

  • Product name

    Rabbit IgG, polyclonal - Isotype Control
    See all Rabbit isotype controls

  • Specificity

    This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details

  • General notes

      

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 8.20
    Constituents: 0.6% Boric Acid, 0.95% Sodium borate, 0.4% Sodium chloride
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Protein A-purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

  • Alternative names

    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab37415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24961933
ICC/IF Use at an assay dependent concentration. PubMed: 23995768
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990

Images

  • Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)
    Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.

    PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.

  • ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)
    ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.

References

This product has been referenced in:

  • D'Onofrio N  et al. MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients. J Cell Physiol 235:1438-1452 (2020). Read more (PubMed: 31294459) »
  • Peng C  et al. BCL6-Mediated Silencing of PD-1 Ligands in Germinal Center B Cells Maintains Follicular T Cell Population. J Immunol 202:704-713 (2019). Read more (PubMed: 30567732) »
See all 80 Publications for this product

Publishing research using ab37415? Please let us know so that we can cite the reference in this datasheet.

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