Rabbit IgG, polyclonal - Isotype Control (ab37415)
Overview
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Product name
Rabbit IgG, polyclonal - Isotype Control
See all Rabbit isotype controls -
Specificity
This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details -
General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Protein A-purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
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Alternative names
- rabbit isotype control
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. PubMed: 24961933
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23995768
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
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| ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20673990
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. PubMed: 24961933 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23995768 |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. |
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ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990 |
Images
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Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.
PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.
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ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.
Protocols
References (84)
ab37415 has been referenced in 84 publications.
- D'Onofrio N et al. MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients. J Cell Physiol 235:1438-1452 (2020). PubMed: 31294459
- Ito S et al. MRTF-A promotes angiotensin II-induced inflammatory response and aortic dissection in mice. PLoS One 15:e0229888 (2020). PubMed: 32208430
- Xie W et al. The H2B ubiquitin-protein ligase RNF40 is required for somatic cell reprogramming. Cell Death Dis 11:287 (2020). PubMed: 32341358
- García-Valero J et al. Deficient pulmonary IFN-ß expression in COPD patients. PLoS One 14:e0217803 (2019). PubMed: 31170225
- Lu W et al. The long non-coding RNA Snhg3 is essential for mouse embryonic stem cell self-renewal and pluripotency. Stem Cell Res Ther 10:157 (2019). PubMed: 31151411
