Rabbit IgG, polyclonal - Isotype Control (ab37415)

Submit a Question Specific References (52)

Overview

  • Product name
    Rabbit IgG, polyclonal - Isotype Control
    See all Rabbit isotype controls
  • Specificity
    This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Protein A-purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas
  • Alternative names
    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab37415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24961933
ICC/IF Use at an assay dependent concentration. PubMed: 23995768
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990

Images

  • Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)
    Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024.

    Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.

    PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.

  • ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)
    ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566.

    ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.

References

This product has been referenced in:
  • Ruan D  et al. XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer. Stem Cell Reports 10:494-508 (2018). Read more (PubMed: 29337117) »
  • Jiang Y  et al. Epigenetic activation during T helper 17 cell differentiation is mediated by Tripartite motif containing 28. Nat Commun 9:1424 (2018). Read more (PubMed: 29651155) »

See all 52 Publications for this product

Publishing research using ab37415? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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