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  1. Link

    rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870.pdf

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Isotype/Loading Controls Isotype Controls Rabbit
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Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

  • Datasheet
  • SDS
Submit a question References (196)

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Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

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Overview

  • Product name

    Rabbit IgG, polyclonal - Isotype Control (ChIP Grade)
    See all Rabbit isotype controls
  • Tested applications

    Suitable for: WB, ChIP, Flow Cytmore details
  • General notes

    For more information regarding the isotype control selection, please see https://www.abcam.com/primary-antibodies/your-guide-to-selecting-an-isotype-control

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches with a concentration less than 1 mg/ml will have 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Rabbit
  • Alternative names

    • rabbit isotype control

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Related Products

    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml.
ChIP
Use a concentration of 1 µg/ml.
Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Notes
WB
Use a concentration of 1 µg/ml.
ChIP
Use a concentration of 1 µg/ml.
Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Images

  • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab171870 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Overlay histogram showing HeLa cells stained with ab75186 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75186, 0.05μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.05μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    Lanes 1-6 : Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) at 1 µg/ml
    Lanes 7-12 : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    Lanes 1 & 7 : Human liver tissue lysate - total protein (ab29889)
    Lanes 2 & 8 : Liver (Mouse) Tissue Lysate
    Lanes 3 & 9 : Liver (Rat) Tissue Lysate
    Lanes 4 & 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lanes 5 & 11 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lanes 6 & 12 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 2 minutes


    Please note that ab171870 in lanes 1-6 represents a negative control for Beta Actin, positively seen in lanes 7-12.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    All lanes : Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Rat Kidney Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Protocols

  • ChIP protocols
  • Flow cytometry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (196)

Publishing research using ab171870? Please let us know so that we can cite the reference in this datasheet.

ab171870 has been referenced in 196 publications.

  • Kobayashi H  et al. The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis. Gastroenterology 160:1224-1239.e30 (2021). PubMed: 33197448
  • Kong NR  et al. Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression. Cell Rep 34:108574 (2021). PubMed: 33406418
  • Sun J  et al. microRNA-27b shuttled by mesenchymal stem cell-derived exosomes prevents sepsis by targeting JMJD3 and downregulating NF-?B signaling pathway. Stem Cell Res Ther 12:14 (2021). PubMed: 33413595
  • Eustaquio Do Imperio G  et al. Function of Multidrug Resistance Transporters is Disrupted by Infection Mimics in Human Brain Endothelial Cells. Tissue Barriers 9:1860616 (2021). PubMed: 33427563
  • Cheng M  et al. Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma. Mol Ther Nucleic Acids 23:418-430 (2021). PubMed: 33473327
View all Publications for this product

Customer reviews and Q&As

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