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    rabbit-igg-vhh-single-domain-hrp-ab191866.pdf

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Secondary antibodies anti-Rabbit IgG Enzyme HRP
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Recombinant

Recombinant Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (92)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
  • Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

Key features and details

  • Anti-Rabbit IgG VHH Single Domain (HRP)
  • Conjugation: HRP
  • Suitable for: WB, IHC-P, ELISA

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Overview

  • Product name

    Anti-Rabbit IgG VHH Single Domain (HRP)
    See all Rabbit IgG secondary antibodies
  • Target species

    Rabbit
  • Specificity

    This antibody is specific to Rabbit IgG VHH Single Domain
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Purified via His tag
  • Purification notes

    This product is a recombinant protein produced in E. coli.
  • Clonality

    Monoclonal
  • Clone number

    GD001
  • Research areas

    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent concentration.
IHC-P (1)
Use a concentration of 0.02 - 0.2 µg/ml.
ELISA
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.
IHC-P
Use a concentration of 0.02 - 0.2 µg/ml.
ELISA
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

    IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to beta tubulin (ab6046, 0.5µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 0.125µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml

    Lanes 1 & 3 : Mouse Brain Tissue Lysate
    Lanes 2 & 4 : Rat Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
    Lanes 3-4 : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 0.05 µg/ml

    Developed using the ECL technique.

    Performed under reducing conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

    IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

    Top left: IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to Ki67 (ab15580, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 0.1µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    Top right: this image shares the same experimental design parameters, except the secondary antibody was ab97051, goat anti-rabbit IgG H&L (HRP) (0.1µg/ml). This demonstrates the improved definition of staining given by VHH Single Domain Antibodies over conventional secondaries.

    Bottom right: this image shares the same experimental design parameters but is a negative control (no primary antibody) for ab97051, demonstrating specificity of the goat anti-rabbit secondary antibody.

    Bottom left: this image shares the same experimental design parameters but is a negative control (no primary antibody) for ab191866, demonstrating the specificity of the VHH-Single Domain Antibody.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

    IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to Ki67 (ab15580, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 0.025µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)

    IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to VDAC1 (ab15895, 1/1000 dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 0.125µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) insert shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    Western blot - Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
    All lanes : Anti-GAPDH antibody - Loading Control (ab37168) at 1 µg/ml

    Lanes 1 & 3 : HeLa Whole Cell Lysate
    Lanes 2 & 4 : Jurkat Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml
    Lanes 3-4 : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 0.02 µg/ml

    Developed using the ECL technique.

    Performed under reducing conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (92)

Publishing research using ab191866? Please let us know so that we can cite the reference in this datasheet.

ab191866 has been referenced in 92 publications.

  • Mohseni R  et al. Therapeutic effects of Chlorella vulgaris on carbon tetrachloride induced liver fibrosis by targeting Hippo signaling pathway and AMPK/FOXO1 axis. Mol Biol Rep 48:117-126 (2021). PubMed: 33296068
  • Tian T  et al. IOX1 protects from TGF-ß induced fibrosis in LX-2 cells via the regulation of extracellular matrix protein expression. Exp Ther Med 21:180 (2021). PubMed: 33488789
  • Saif Z  et al. A preferential switch between placental GR exon 1 promoter variants in the presence of maternal asthma or inflammation upregulates GRa D isoforms. Placenta 108:64-72 (2021). PubMed: 33819863
  • Wang C  et al. Oncogenic roles of the cholesterol metabolite 25-hydroxycholesterol in bladder cancer. Oncol Lett 19:3671-3676 (2020). PubMed: 32382321
  • Quirino-Teixeira AC  et al. Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1. Blood Adv 4:2018-2031 (2020). PubMed: 32396616
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

IHC staining of Beta-tubulin III in mouse nerve using anti-rabbit IgG VHH Single Domain_HRP as 2nd antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The mouse sciatic nerve paraffin sections was dewaxed and rehydrated. Prior to IHC detection, the sections was pre-treated by steaming in 10 mM citrate buffer (pH6.0) for 20 min. Protein non-specific binding was blocked by incubated in 10% goat sera plus 2xcasin for 30min. The sections were then incubated with rabbit anti-beta tubulin III (ab18207) for 1.5 hr at room temp (at 1:1,000 in PBS plus 1xcasein). After washing in PBST (0.05% Tween 20) two times, 5 min each, the sections were incubated with the 2nd antibody, anti-rabbit IgG VHH Single Domain_HRP (ab191866) for 30 min at room temp. After washing in PBST two times, 5 min each, the sections were proceeded with color development with Nova Red as chromogen and then counterstained briefly with hematoxylin and mounted with Permount.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Sep 08 2015

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