Key features and details
- Sensitivity: 0.042 ng/ml
- Range: 0.041 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Product nameRabbit IL-13 ELISA Kit
See all IL-13 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.041 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Serum 89.69 81% - 97% Plasma 75.91 71% - 80% Cell culture media 95.33 87% - 102%
Assay durationMultiple steps standard assay
Rabbit IL-13 ELISA Kit (ab277391) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit IL-13 in serum (Rabbit IL-13 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and Rabbit IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Rabbit IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Rabbit IL-13 Antibody 2 vials Anti-Rabbit IL-13 coated Microplate 1 unit Rabbit IL-13 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- Allergic rhinitis
- BHR 1
ab277391 has not yet been referenced specifically in any publications.