Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Product nameRabbit IL-1RA ELISA Kit
See all IL-1RA kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.205 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Serum 75.95 69% - 88% Plasma 83.97 70% - 95% Cell culture media 89.8 83% - 95%
Assay durationMultiple steps standard assay
Rabbit IL-1RA ELISA Kit (ab277394) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit IL-1RA in serum (Rabbit IL-1ra concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit IL-1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and Rabbit IL-1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit IL-1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Rabbit IL-1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated Anti-Rabbit IL-1 RA Antibody 2 vials Anti-Rabbit IL-1RA coated Microplate 1 unit Rabbit IL-1RA standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cellular localizationCytoplasm and Secreted.
- Information by UniProt
- ICIL 1RA
ab277394 has not yet been referenced specifically in any publications.