Rabbit Loading Control Antibody Panel (ab199712)

Submit a review Submit a question References (384)
Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)
  • Western blot - Anti-Histone H3 antibody [EPR16987] (ab176842)
  • Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • Rabbit Loading Control Antibody Panel (ab199712)

Overview

  • Product name

    Rabbit Loading Control Antibody Panel
    See all Loading Control Panel kits

  • Product overview

    Ab199712 is a sampler pack of loading control antibodies and a Goat Anti-Rabbit IgG secondary antibody conjugated to HRP.
    This panel contains sample sizes of primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, COXIV, Histone H3, and NaK ATPase.


    The Rabbit Loading Control Antibody Panel is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Refer to 'Associated Products' section below to see HRP-conjugated versions of these antibodies.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
    Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)

    All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/50000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Xenopus(X. tropicalis) muscle lysates
    Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 50 kDa

    Secondary antibodies - anti-rabbit HRP (ab6721)

  • Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)
    Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    All lanes : Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554) at 1/10000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H3 antibody [EPR16987] (ab176842)
    Western blot - Anti-Histone H3 antibody [EPR16987] (ab176842)

    All lanes : Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade (ab176842) at 1 µg

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Drosophila embryo nuclear extract (from melanogaster embryos 0-12hr) at 10 µg
    Lane 4 : S.cerevisiae (Y190) Whole Cell Lysate at 20 µg
    Lane 5 : S.pombe Whole Cell Lysate at 20 µg

    Secondary
    Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab176842 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
    Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)

    All lanes : Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020) at 1/100000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size : 113 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Rabbit Loading Control Antibody Panel (ab199712)
    Rabbit Loading Control Antibody Panel (ab199712)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (384)

Publishing research using ab199712? Please let us know so that we can cite the reference in this datasheet.

ab199712 has been referenced in 384 publications.

  • Lojewski X  et al. Human iPSC models of neuronal ceroid lipofuscinosis capture distinct effects of TPP1 and CLN3 mutations on the endocytic pathway. Hum Mol Genet 23:2005-22 (2014). PubMed: 24271013
  • Gurbuz I  et al. SAP domain-dependent Mkl1 signaling stimulates proliferation and cell migration by induction of a distinct gene set indicative of poor prognosis in breast cancer patients. Mol Cancer 13:22 (2014). PubMed: 24495796
  • McGoldrick CA  et al. Identification of oxidized protein hydrolase as a potential prodrug target in prostate cancer. BMC Cancer 14:77 (2014). PubMed: 24512522
  • Mayle S & Monie TP The nucleotide-binding oligomerization domain-containing protein 1 (NOD1) polymorphism S7N does not affect receptor function. BMC Res Notes 7:124 (2014). PubMed: 24598002
  • Juhász T  et al. Pituitary adenylate cyclase activating polypeptide (PACAP) signalling exerts chondrogenesis promoting and protecting effects: implication of calcineurin as a downstream target. PLoS One 9:e91541 (2014). PubMed: 24643018
View all Publications for this product

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