Recombinant Anti-PIM1 antibody [EPR24447-39] (ab300453)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24447-39] to PIM1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PIM1 antibody [EPR24447-39]
See all PIM1 primary antibodies -
Description
Rabbit monoclonal [EPR24447-39] to PIM1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt (Intra) or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K-562, K-562 transfected with siRNA specifically targeti PIM1, whole, K-562, A20, F9, His-tagged human PIM1 recombinant protein and His-tagged human PIM3 recombinant protein lysates. IP: A20 cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24447-39 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
Tested in mouse samples only |
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WB |
1/1000. Detects a band of approximately 34, 44 kDa.
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Notes |
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IP
1/30. Tested in mouse samples only |
WB
1/1000. Detects a band of approximately 34, 44 kDa. |
Target
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Function
May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. -
Tissue specificity
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on both serine/threonine and tyrosine residues. -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5292 Human
- Entrez Gene: 18712 Mouse
- Omim: 164960 Human
- SwissProt: P11309 Human
- SwissProt: P06803 Mouse
- Unigene: 81170 Human
- Unigene: 405293 Mouse
- Unigene: 485038 Mouse
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Alternative names
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
see all
Images
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All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/5000 dilution
Lane 1 : His-tagged human PIM1 recombinant protein
Lane 2 : His-tagged human PIM3 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 34,44 kDa why is the actual band size different from the predicted?
Exposure time: 7 seconds -
All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : A20 (Mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 3 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking / Diluting buffer and concentration:5% NFDM/TBST
Exposure time:
Lane 1, 2: 70 seconds
Lane 3: 3 minutes -
All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : K-562 transfected with siRNA specifically targeting PIM1, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
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PIM1 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug with ab300453 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300453 at ID63 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug
Lane 2: ab300453 IP in A20 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300453 in A20 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300453 has not yet been referenced specifically in any publications.