Recombinant Anti-CD39 antibody [EPR26473-58] (ab300065)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26473-58] to CD39
- Suitable for: WB, Flow Cyt, IP, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD39 antibody [EPR26473-58]
See all CD39 primary antibodies -
Description
Rabbit monoclonal [EPR26473-58] to CD39 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IP, IHC-P, ICC/IFmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate, THP-1 (Human monocytic leukemia monocyte) whole cell lysate, Jurkat ( Human T cell leukemia T lymphocyte) whole cell lysate, Human placenta tissue lysate, Human heart tissue lysate. IHC-P: Human : endometrial carcinoma, pancreas, liver tissue. ICC/IF: Human PBMC cells, IM-9 (human multiple myeloma B Lymphoblast) cells,. Flow cyt.: Human peripheral blood mononuclear cell (PBMC) cells. IP: Human tissue lysates of heart and placenta.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26473-58 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 57 kDa).
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Flow Cyt |
1/500.
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IP |
1/30.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/50.
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Notes |
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WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 57 kDa). |
Flow Cyt
1/500. |
IP
1/30. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
Target
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Function
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
Tissue specificity
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsThe N-terminus is blocked.
Palmitoylated in the N-terminal part. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 953 Human
- Omim: 601752 Human
- SwissProt: P49961 Human
- Unigene: 576612 Human
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Form
There are 3 isoforms produced by alternative splicing. -
Alternative names
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
Images
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All lanes : Anti-CD39 antibody [EPR26473-58] (ab300065) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?5% NFDM/TBST was used as blocking and diluting buffer.
Exposure time:
Lane 1:59 seconds
Lane 2:180 seconds -
All lanes : Anti-CD39 antibody [EPR26473-58] (ab300065) at 1/1000 dilution
Lane 1 : IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Jurkat ( Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?5% NFDM/TBST was used as blocking and diluting buffer.
Exposure time:
Lane 1: 10 seconds
Lane 2 and 3: 103 secondsNegative control: Jurkat ( PMID:9077545).
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD39 with ab300065 at 1/2000 (0.295 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on the endothelial cells and smooth muscle of human liver.The section was incubated with ab300065 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling with ab300065 at 1/2000 (0.295 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on the endothelial cells and smooth muscle of human pancreas.The section was incubated with ab300065 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling CD39 with ab300065 at 1/2000 (0.295 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on the stromal cells of human endometrial carcinoma (PMID: 25403716).The section was incubated with ab300065 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeilized IM-9 (human multiple myeloma B Lymphoblast) cells lebelling CD39 with ab300065 at 1/50 (11.8 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/mL) dilution (Green). Confocal image showing membranous and cytoplasmic staining in IM-9 cell line. Negative control: Jurkat (PMID: 24319640). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/mL) dilution.
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Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeilized Human PBMC cells lebelling CD39 with ab300065 at 1/50 (11.8 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/mL) dilution (Green). Confocal image showing membranous and cytoplasmic staining in human PBMC is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD39 with ab300065 at 1/500 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Cells were stained with rabbit IgG or ab300065. Then stained with anti-CD19 conjugated to Alexa Fluor®647.
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CD39 was immunoprecipitated from 0.35 mg Human placenta tissue lysate 10µg with ab300065 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300065 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human placenta tissue lysate 10µg
Lane 2: ab300065 in Human placenta tissue lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab300065 in Human placenta tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
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CD39 was immunoprecipitated from 0.35 mg Human heart tissue lysate 10µg with ab300065 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300065 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human heart tissue lysate 10µ
Lane 2: ab300065 in Human heart tissue lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab300065 in Human heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 67 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300065 has not yet been referenced specifically in any publications.