Product nameRabbit monoclonal [EPR4418] Anti-Human IgG2 H&L (HRP)
See all IgG2 secondary antibodies
DescriptionRabbit monoclonal [EPR4418] Anti-Human IgG2 H&L (HRP)
SpecificityDoes not cross-react with Human IgG1, IgG3 or IgG4.
Tested applicationsSuitable for: WB, IHC-Pmore details
Synthetic peptide within Human IgG2 aa 50-150. The exact sequence is proprietary.
Database link: P01859
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab236478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 36 kDa).|
|IHC-P||1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
IHC image of IgG2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab236478, 1/250 dilution, for 15 minutes at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
All lanes : Rabbit monoclonal [EPR4418] Anti-Human IgG2 H&L (HRP) (ab236478) at 1/5000 dilution
Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue
Lane 2 : Tonsil (Human) Whole Cell Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab236478 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab236478 has not yet been referenced specifically in any publications.