Recombinant
RabMAb

Recombinant Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)

Overview

  • Product name

    Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L
    See all IgG1 secondary antibodies
  • Description

    Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L
  • Host species

    Rabbit
  • Target species

    Human
  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG1. Immunizing IgG digested fragments

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.005% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    H26-10
  • Isotype

    IgG
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Images

  • Direct ELISA antibody dose-response curve using ab125912 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Direct ELISA antibody dose-response curve using ab125912 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

References

ab125912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


I am sorry to confirm that we do not have an HRP conjugated version of the clone available.

There is the following alternative in the catalog which is tested in ELISA:


Mouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (HRP) (ab99774)
https://www.abcam.com/index.html?datasheet=99774 (or use the following: https://www.abcam.com/index.html?datasheet=99774).

Or you may like to consider our conjugation kits to conjugate ab125912 with HRP:

HRP Conjugation Kit (ab102890)
https://www.abcam.com/index.html?datasheet=102890 (or use the following: https://www.abcam.com/index.html?datasheet=102890).

When using the conjugation kits I can recommend to review the frequently asked questions page for more information regardign compatability etc.

https://www.abcam.com/index.html?pageconfig=resource&rid=13156


We also have the following ELISA kit which may be useful to consider:

Human IgG ELISA Kit SimpleStep (ab195215)
Description: Human IgG ELISA Kit SimpleStep
Reactivity: Human
Sample type: Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Lysate

https://www.abcam.com/index.html?datasheet=195215 (or use the following: https://www.abcam.com/index.html?datasheet=195215).

The sensitivity of this kit is Sensitivity 0.02 ng/ml

Read More

Answer

Thank you for your inquiry.
The lab has not determined the dissociation constant for this antibody, however rabbit monoclonals typically have very high affinities (typically in the picomolar level).
Minimum: 10^-9 - 10^-10 KDM
Maximum: 10^-12 KDM
I hope this information helps. Please let me know if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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