Recombinant Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
Key features and details
- Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin)
See all IgG1 secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: IHC-P, ELISAmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
H26-10 -
Isotype
IgG -
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ELISA |
Use a concentration of 0 - 100 µg/ml.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
Use a concentration of 0 - 100 µg/ml. |
Images
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Ab201485 was tested using a sandwich ELISA approach. The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
Negative IHC image of Human IgG1 H & L staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab201485, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
IHC image of Human IgG1 H & L staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab201485, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab201485 has been referenced in 2 publications.
- Jia H et al. Actin-binding protein anillin promotes the progression of hepatocellular carcinoma in vitro and in mice. Exp Ther Med 21:454 (2021). PubMed: 33747188
- Yue FR et al. SMYD3 promotes colon adenocarcinoma (COAD) progression by mediating cell proliferation and apoptosis. Exp Ther Med 20:11 (2020). PubMed: 32934676