RecombinantRabMAb

Recombinant Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)

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Key features and details

  • Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ELISA

Overview

  • Product name

    Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Mouse

  • Tested applications

    Suitable for: ELISAmore details

  • Immunogen

    Other Immunogen Type corresponding to IgG.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.005% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    M111-2

  • Isotype

    IgG

  • General notes

    Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Images

  • ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)
    ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)

    Direct ELISA antibody dose-response curve using ab125904 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab125904? Please let us know so that we can cite the reference in this datasheet.

ab125904 has been referenced in 3 publications.

  • Weber J  et al. PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. Nat Commun 10:1415 (2019). PubMed: 30926791
  • Klaus T & Bereta J CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination. Front Immunol 9:1096 (2018). PubMed: 29875771
  • Klaus T  et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep 6:30938 (2016). ELISA . PubMed: 27484487

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

j’aurais aimé savoir si cet anticorps est Fc spécifique car cela n’est pas précisé dans la datasheet.

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Answer

Malheureusement nous n'avons pas les données sur si ab125904 est FC spécifique. Nous avons uniquement confirmé qu'il reconnait spécifiquement Mouse IgG1, IgG2a, IgG3.

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Question

what is the binding affinity for these antibodies?

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Answer

Thank you for contacting us.

We do not measure the binding affinity of each antibody at this time, however, we may start providing binding affinities in the future. With RabMAbs, typical affinities are in the 10^-9 to 10^-10 Kd (M) range. Affinites in the picomolar range (10^-12 Kd (M)) are possible.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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