RecombinantRabMAb

Recombinant Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)

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ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)

    Key features and details

    • Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: ELISA

    Conjugates logo Related conjugates and formulations

    Carrier Free

    Overview

    • Product name

      Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3
      See all IgG secondary antibodies

    • Host species

      Rabbit

    • Target species

      Mouse

    • Tested applications

      Suitable for: ELISAmore details

    • Immunogen

      Other Immunogen Type corresponding to IgG.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.2
      Preservative: 0.005% Sodium azide
      Constituents: 0.1% BSA, PBS
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      M111-2

    • Isotype

      IgG

    • General notes

      Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab125904 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/1000.
    Notes
    ELISA
    1/1000.

    Images

    • ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)
      ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 (ab125904)

      Direct ELISA antibody dose-response curve using ab125904 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab125904? Please let us know so that we can cite the reference in this datasheet.

    ab125904 has been referenced in 3 publications.

    • Weber J  et al. PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. Nat Commun 10:1415 (2019). PubMed: 30926791
    • Klaus T & Bereta J CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination. Front Immunol 9:1096 (2018). PubMed: 29875771
    • Klaus T  et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep 6:30938 (2016). ELISA . PubMed: 27484487

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    j’aurais aimé savoir si cet anticorps est Fc spécifique car cela n’est pas précisé dans la datasheet.

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    Answer

    Malheureusement nous n'avons pas les données sur si ab125904 est FC spécifique. Nous avons uniquement confirmé qu'il reconnait spécifiquement Mouse IgG1, IgG2a, IgG3.

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    Question

    what is the binding affinity for these antibodies?

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    Answer

    Thank you for contacting us.

    We do not measure the binding affinity of each antibody at this time, however, we may start providing binding affinities in the future. With RabMAbs, typical affinities are in the 10^-9 to 10^-10 Kd (M) range. Affinites in the picomolar range (10^-12 Kd (M)) are possible.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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