Recombinant
RabMAb

Recombinant Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)

Overview

  • Product name

    Rabbit monoclonal [R18-2] Anti-Rat IgG Fc
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Rat
  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    R18-2
  • Isotype

    IgG
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.0156 - 1 µg/ml.

Images

  • Direct ELISA antibody dose-response curve using ab125900 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Antibody dose-response curve using purified ab125900. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

References

This product has been referenced in:

  • de Castro Martin IF  et al. Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles. Nat Commun 8:1396 (2017). Read more (PubMed: 29123131) »
  • Wittenborn TR  et al. Simulation of heterogeneous molecular delivery in tumours using µCT reconstructions and MRI validation. Microvasc Res 108:69-74 (2016). Read more (PubMed: 27569845) »
See all 4 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you very much for your call last week and for your patience while I've been in touch with the lab regarding your enquiry.
The immunogen was whole rat IgG, but different subclones were generated and then were tested for specificity against F(ab) or Fc fragments by ELISA.

I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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