Key features and details
- Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ICC, ELISA
Product nameRabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)
See all Kappa light chain secondary antibodies
ab238618 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of ab238618 has been engineered to eliminate Fc receptor binding.
Tested applicationsSuitable for: WB, ICC, ELISAmore details
Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Light chain typekappa
Our Abpromise guarantee covers the use of ab238618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 25 kDa.|
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
|ELISA||Use a concentration of 0.005 - 2 µg/ml.|
A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
All lanes : Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618) at 0.2 µg/ml
Lanes 1 & 3 : Non-reduced(-) mouse IgG1?
Lanes 2 & 4 : Reduced(+) mouse IgG1?
Predicted band size: 25 kDa
This antibody reacts to non-reduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
ELISA of mouse immunoglobulins shows ab238618 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/ml, 50 ng/ml, or 10 ng/ml of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238618 has not yet been referenced specifically in any publications.