Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)

Overview

  • Product name

    Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)
    See all Kappa light chain secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    ab238618 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of ab238618 has been engineered to eliminate Fc receptor binding.

  • Tested applications

    Suitable for: WB, ICC, ELISAmore details
  • Immunogen

    Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM103
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab238618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 25 kDa.
ICC Use a concentration of 0.5 - 2 µg/ml.
ELISA Use a concentration of 0.005 - 2 µg/ml.

Images

  • A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • All lanes : Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618) at 0.2 µg/ml

    Lanes 1 & 3 : Non-reduced(-) mouse IgG1?
    Lanes 2 & 4 : Reduced(+) mouse IgG1?

    Predicted band size: 25 kDa



    This antibody reacts to non-reduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).

  • ELISA of mouse immunoglobulins shows ab238618 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/ml, 50 ng/ml, or 10 ng/ml of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab238618 has not yet been referenced specifically in any publications.

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