Key features and details
- Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Product nameRabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
See all IgG1 secondary antibodies
ab238623 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
The Fc region of ab238623 has been engineered to eliminate Fc receptor binding.
Tested applicationsSuitable for: WB, ELISAmore details
Full length protein corresponding to Mouse IgG1. (Mouse IgG).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 5 µg/ml. Predicted molecular weight: 36 kDa.|
|ELISA||Use a concentration of 0.005 - 2 µg/ml.|
All lanes : Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623) at 0.2 µg/ml
Lane 1 : Non-reduced (-) mouse IgG1, 20 ng/lane
Lane 2 : Reduced (+) mouse IgG1, 20 ng/lane
Predicted band size: 36 kDa
This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of mouse immunoglobulins shows ab238623 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238623 has not yet been referenced specifically in any publications.