Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)

Overview

  • Product name

    Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
    See all IgG1 secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    ab238623 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

    The Fc region of ab238623 has been engineered to eliminate Fc receptor binding.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Full length protein corresponding to Mouse IgG1. (Mouse IgG).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM106
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab238623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ELISA Use a concentration of 0.005 - 2 µg/ml.

Images

  • All lanes : Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623) at 0.2 µg/ml

    Lane 1 : Non-reduced (-) mouse IgG1, 20 ng/lane
    Lane 2 : Reduced (+) mouse IgG1, 20 ng/lane

    Predicted band size: 36 kDa



    This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).

  • A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA of mouse immunoglobulins shows ab238623 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab238623 has not yet been referenced specifically in any publications.

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