Recombinant

Recombinant Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)

Overview

  • Product name

    Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    ab238532 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
    The Fc region of RM108 has been engineered to eliminate Fc receptor binding.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Full length protein corresponding to Mouse IgG2b.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab238532 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM108
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab238532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.
ELISA Use a concentration of 0.005 - 0.2 µg/ml.

Images

  • All lanes : Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532) at 0.2 µg/ml

    Lane 1 : Non-reduced mouse IgG2b
    Lane 2 : Reduced mouse IgG2b

    Lysates/proteins at 20 µg per lane.
  • ELISA of mouse immunoglobulins shows ab238532 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab238532 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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