Key features and details
- Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Product nameRabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)
ab238532 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
Tested applicationsSuitable for: WB, ELISAmore details
Full length protein corresponding to Mouse IgG2b.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesab238532 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
All lanes : Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532) at 0.2 µg/ml
Lane 1 : Non-reduced mouse IgG2b
Lane 2 : Reduced mouse IgG2b
Lysates/proteins at 20 µg per lane.
ELISA of mouse immunoglobulins shows ab238532 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238532 has not yet been referenced specifically in any publications.