Key features and details
- Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, Sandwich ELISA
Product nameRabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
See all IgG4 secondary antibodies
ab238617 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Tested applicationsSuitable for: IHC-P, WB, Sandwich ELISAmore details
Synthetic peptide corresponding to Human IgG4 (Hinge).
Database link: P01861
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 36 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration.
Use 50ng/well – 200ng/well (for Capture); 0.05µg/mL – 0.2µg/mL (for Detection).
Sandwich ELISA using ab238617 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
Sandwich ELISA using ab238617 as the capture antibody, and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
Paraffin-embedded human lymphoid tissue stained for IgG4 heavy chain using ab238617 at 10 μg/mL in immunohistochemical analysis.
A titer ELISA using ab238617. The plate was coated with different amounts of human IgG4. A serial dilution of ab238617 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
All lanes : Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617) at 2 µg/ml
Lane 1 : Non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : Non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : Non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : Non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : Non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : Non-reduced (-) and reduced (+) forms of Rat IgG
Lane 7 : Non-reduced (-) and reduced (+) forms of Goat IgG
Predicted band size: 36 kDa
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms (Lane 4). No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238617 has not yet been referenced specifically in any publications.