Overview

  • Product name

    Rabbit monoclonal [RM218] Anti-Mouse IgG3 F(ab)
    See all IgG3 secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    ab238616 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.

  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Full length protein corresponding to Mouse IgG3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM218
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.005 - 0.2 µg/ml.

Images

  • A titer ELISA of mouse IgG3.

    The plate was coated with different amounts of mouse IgG3.

    A serial dilution of ab238616 was used as the primary antibody.

    An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA of mouse immunoglobulins shows ab238616 reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238616 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab238616 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab238616.
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