Key features and details
- Rabbit monoclonal [RM218] Anti-Mouse IgG3 F(ab)
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
Product nameRabbit monoclonal [RM218] Anti-Mouse IgG3 F(ab)
See all IgG3 secondary antibodies
ab238616 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Tested applicationsSuitable for: ELISAmore details
Full length protein corresponding to Mouse IgG3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
A titer ELISA of mouse IgG3.
The plate was coated with different amounts of mouse IgG3.
A serial dilution of ab238616 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of mouse immunoglobulins shows ab238616 reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238616 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238616 has not yet been referenced specifically in any publications.