Key features and details
- Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, ICC
Product nameRabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
See all IgG2c secondary antibodies
ab238619 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Tested applicationsSuitable for: WB, ELISA, ICCmore details
Full length protein corresponding to Mouse IgG2c.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.2 µg/ml.|
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
All lanes : Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619) at 0.5 µg/ml
Lane 1 : Non-reduced (-) mouse IgG2c
Lane 2 : Reduced (+) mouse IgG2c
This antibody reacts to non-reduced IgG2c.
A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of ab238619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of IgG from BalB/C and C57BL6 shows ab238619 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab2308619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238619 has not yet been referenced specifically in any publications.