Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619)

Overview

  • Product name

    Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
    See all IgG2c secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    ab238619 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

  • Tested applications

    Suitable for: WB, ELISA, ICCmore details
  • Immunogen

    Full length protein corresponding to Mouse IgG2c.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM223
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml.
ELISA Use a concentration of 0.01 - 0.2 µg/ml.
ICC Use a concentration of 0.5 - 2 µg/ml.

Images

  • All lanes : Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619) at 0.5 µg/ml

    Lane 1 : Non-reduced (-) mouse IgG2c
    Lane 2 : Reduced (+) mouse IgG2c


    This antibody reacts to non-reduced IgG2c.

  • A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of ab238619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA of IgG from BalB/C and C57BL6 shows ab238619 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab2308619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab238619 has not yet been referenced specifically in any publications.

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