Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L
  • Host species
    Rabbit
  • Target species
    Mouse
  • Specificity
    Recognizes mouse IgG heavy and light chains.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Native, mouse whole IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was purified by affinity chromatography using mouse IgG-agarose.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000.

This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.

References

This product has been referenced in:
  • O'Geen H  et al. Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. PLoS One 13:e0193818 (2018). Read more (PubMed: 29584750) »
  • Verhoeven RJA  et al. NF-?B Signaling Regulates Epstein-Barr Virus BamHI-Q-Driven EBNA1 Expression. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 29659505) »

See all 110 Publications for this product

Publishing research using ab46540? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 reviews or Q&A

10
Votes: Highest First

I need some help designing my ChIP experiment.

Thank you for contacting us.


First, I'd like to point out some resources on our website which may help:


https://www.abcam.com/index.html?rid=310


A Beginner's Guide to ChIP - Abcam


As we di...

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phone call about rabbit IGG controls for ChIP

Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouv&...

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Hello,

I'm using Rabbit Control IgG - ChIP Grade (ab46540)
for my chip experiment.
Do you have equivalent for rat and mouse? I don't find them,


Thanks in advance,

Thank you for your inquiry.

Indeed we do have also polyclonal control fractions of mouse or rat IgGs.

For the mouse IgG, we do have the IgG fraction ab37355 https://www.abcam.com/index.html?datasheet=37355 (or use the following: htt...

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dear correspondent,

We have lately purchased several Abs from Abcam and i have a question related to the purification method.
In the datasheets of several monoclonal antibodies is mentioned that they are purified as 'IgG fraction'. Coul...

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Thank you for your enquiry.

I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

ab3609 (Aurora B)
Protein G purified

ab51841 (TATA binding protein...

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Dear, We lately bought the rabbit control IgG antibody. We would like to rebuy it but need further information on the following question. Is this antibody purified via protein A chromatography or not? This is important information for us if we want to ...

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Thank you for your inquiry.
I can confim that the antibody was affinity-purified against the antigen itself (bound to agarose).
Protein A or G was not used.
I hope this information is helpful. please do not hesitate to contact me with an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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