Rabbit Anti-Mouse IgG H&L (ab6709)
Key features and details
- Rabbit Anti-Mouse IgG H&L
- Host species: Rabbit
- Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
Immunogen
Mouse IgG, whole molecule
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
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| IP |
Use at an assay dependent concentration.
|
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| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| ELISA |
1/1.6e+006.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
ELISA
1/1.6e+006. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
Images
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ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (17)
ab6709 has been referenced in 17 publications.
- Ji Q et al. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation. Nat Commun 11:1211 (2020). PubMed: 32139701
- Sa R et al. MiR-616 plays oncogenic role in hepatocellular carcinoma progression through suppressing PTEN expression and activating PI3K/AKT pathway. Artif Cells Nanomed Biotechnol 48:728-734 (2020). PubMed: 32233811
- Sun H et al. Downregulation of lncRNA H19 alleviates atherosclerosis through inducing the apoptosis of vascular smooth muscle cells. Mol Med Rep 22:3095-3102 (2020). PubMed: 32945413
- He T et al. Inhibition of two-pore channels in antigen-presenting cells promotes the expansion of TNFR2-expressing CD4+Foxp3+ regulatory T cells. Sci Adv 6:N/A (2020). PubMed: 32998896
- Crawley-Snowdon H et al. Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Nat Commun 11:5031 (2020). PubMed: 33024112
