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  1. Link

    rabbit-mouse-igg-hl-ab6709.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Mouse IgG H&L (ab6709)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (33)

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Abpromise

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ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)

    Key features and details

    • Rabbit Anti-Mouse IgG H&L
    • Host species: Rabbit
    • Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Rabbit Anti-Mouse IgG H&L
      See all IgG secondary antibodies
    • Host species

      Rabbit
    • Target species

      Mouse
    • Tested applications

      Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details
    • Immunogen

      Mouse IgG, whole molecule

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads
    • Clonality

      Polyclonal
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Mouse
      • IgG
      • Unconjugated

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6709 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000 - 1/10000.
    IP
    Use at an assay dependent concentration.
    Immunodiffusion
    Use at an assay dependent concentration.
    Conjugation
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/20000 - 1/400000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    WB
    1/2000 - 1/10000.
    IP
    Use at an assay dependent concentration.
    Immunodiffusion
    Use at an assay dependent concentration.
    Conjugation
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/20000 - 1/400000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.

    Images

    • ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
      ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)

      Sensitivity of ab6709 against mouse IgG.

      The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (33)

    Publishing research using ab6709? Please let us know so that we can cite the reference in this datasheet.

    ab6709 has been referenced in 33 publications.

    • Wang X  et al. Antibody-free profiling of transcription factor occupancy during early embryogenesis by FitCUT&RUN. Genome Res 32:378-388 (2022). PubMed: 34965941
    • Garcia-Martin R  et al. Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism. Cell Rep 38:110277 (2022). PubMed: 35045290
    • van Schaik T  et al. Genome-Wide Mapping and Microscopy Visualization of Protein-DNA Interactions by pA-DamID. Methods Mol Biol 2458:215-229 (2022). PubMed: 35103970
    • Feng L  et al. Mechanism of RNA-binding protein Lin28 in neuronal ferroptosis after intracerebral haemorrhage. Folia Neuropathol 60:35-47 (2022). PubMed: 35359144
    • Wang Z  et al. Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation. Mol Cancer 21:92 (2022). PubMed: 35366893
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Do you have the isoelectric point for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 15 2004

    Answer

    Sorry, we don't have that information.

    Read More

    Abcam Scientific Support

    Answered on Jun 23 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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