Rabbit Anti-Mouse IgG H&L (ab6709)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameRabbit Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host speciesRabbit
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Target speciesMouse
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Tested applicationsSuitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details
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Immunogen
Mouse IgG, whole molecule
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads
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ClonalityPolyclonal
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at an assay dependent concentration. | |
| Immunodiffusion | Use at an assay dependent concentration. | |
| Conjugation | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| ELISA | 1/1.6e+006. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Images
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ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
Datasheets and documents
References
This product has been referenced in:
- Xie H & Wang H PRL-3 promotes breast cancer progression by downregulating p14ARF-mediated p53 expression. Oncol Lett 15:2795-2800 (2018). Read more (PubMed: 29435006) »
- Yuan W et al. miR-494 inhibits cell proliferation and metastasis via targeting of CDK6 in osteosarcoma. Mol Med Rep 16:8627-8634 (2017). Read more (PubMed: 28990071) »
