Rabbit Anti-Mouse IgG H&L (ab6709)

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Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • Immunogen

    Mouse IgG, whole molecule

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Conjugation Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
ELISA 1/1.6e+006.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
    ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)

    Sensitivity of ab6709 against mouse IgG.

    The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.

References

This product has been referenced in:
  • Xie H & Wang H PRL-3 promotes breast cancer progression by downregulating p14ARF-mediated p53 expression. Oncol Lett 15:2795-2800 (2018). Read more (PubMed: 29435006) »
  • Feng J  et al. Silencing of Annexin A1 suppressed the apoptosis and inflammatory response of preeclampsia rat trophoblasts. Int J Mol Med 42:3125-3134 (2018). Read more (PubMed: 30272262) »
See all 9 Publications for this product

Publishing research using ab6709? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Do you have the isoelectric point for this antibody?

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Answer

Sorry, we don't have that information.

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