Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729)


  • Product name
    Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    Alkaline Phosphatase


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
  • General notes

    Store secondary antibody at 4° C before opening. DO NOT FREEZE. Mouse conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

  • Research areas


Our Abpromise guarantee covers the use of ab6729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.
WB Use at an assay dependent dilution.


This product has been referenced in:
  • Jang J  et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). Read more (PubMed: 29739951) »
  • Xu F  et al. Modified human glucagon-like peptide-1 (GLP-1) produced in E. coli has a long-acting therapeutic effect in type 2 diabetic mice. PLoS One 12:e0181939 (2017). Read more (PubMed: 28750064) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for contacting us for technical support with ab6729. I think the problem you are experiencing is due to the fact that budding yeast expresses an endogenous form of alkaline phosphate (http://www.expasy.ch/uniprot/O60109) and by using a se...

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I have heard back from the originator of this product and would like to pass along the following comments to you (possible resaons why the AP loss activity). a). Researcher diluted the product and stored at 4oC. THE PRODUCT HAS TO BE STORED UNDILUT...

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Thank you for your email. I have contacted the originator of this antibody in order to see if they have any suggestions or have heard of any problems with the particular lot that you have received. I will not hear back until next week and I'm also goin...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab6729. Can you please tell me the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used?

Thank you for the details that you have provided. I'm surprised that you are unable to see a signal with ab8226 except for the faint signal detected with the dilution of 1:250. We have had some good feedback from customers who have successfully used th...

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Thanks for your email and I'm sorry to hear that you are experiencing difficulty with ab8226. To better assist you, can you please provide me with me with more details regarding your protocol? Also, please include the lot number that you received (i...

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