Product nameRabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Mouse IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Do Not Freeze. Please see notes section. Store undiluted.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Our Abpromise guarantee covers the use of ab6729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|WB||Use at an assay dependent dilution.|
This product has been referenced in:
- Nunes C et al. Red wine extract preserves tight junctions in intestinal epithelial cells under inflammatory conditions: implications for intestinal inflammation. Food Funct 10:1364-1374 (2019). Read more (PubMed: 30735221) »
- Jang J et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). Read more (PubMed: 29739951) »