Product nameRabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Mouse IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Store secondary antibody at 4° C before opening. DO NOT FREEZE. Mouse conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Our Abpromise guarantee covers the use of ab6729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|WB||Use at an assay dependent dilution.|
This product has been referenced in:
- Jang J et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). Read more (PubMed: 29739951) »
- Xu F et al. Modified human glucagon-like peptide-1 (GLP-1) produced in E. coli has a long-acting therapeutic effect in type 2 diabetic mice. PLoS One 12:e0181939 (2017). Read more (PubMed: 28750064) »