Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6727 has been referenced in 2 publications.
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
- Han T et al. HIF-1a contributes to tube malformation of human lymphatic endothelial cells by upregulating VEGFR-3. Int J Oncol 54:139-151 (2019). PubMed: 30431105