Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, IHC-P, WB
Product nameRabbit Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Conjugation notesBiotin/ antibody protein ratio is 4:1
Our Abpromise guarantee covers the use of ab97044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/400000. (Primary).|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||1/500 - 1/5000.|
|WB||1/20000 - 1/400000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97044 has been referenced in 2 publications.
- Zhou M et al. Spontaneous hypothermia ameliorated inflammation and neurologic deficit in rat cardiac arrest models following resuscitation. Mol Med Rep 17:2127-2136 (2018). PubMed: 29207113
- Cottle DL et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291