Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes

    Biotin/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/400000. (Primary).
ICC/IF 1/200 - 1/1000.
IHC-P 1/500 - 1/5000.
WB 1/20000 - 1/400000. (Chemiluminescent).

References

This product has been referenced in:

  • Zhou M  et al. Spontaneous hypothermia ameliorated inflammation and neurologic deficit in rat cardiac arrest models following resuscitation. Mol Med Rep 17:2127-2136 (2018). Read more (PubMed: 29207113) »
  • Cottle DL  et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). Read more (PubMed: 23403291) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

While it is theoretically possible to amplify your signal by using a biotinylated secondary antibody with an anti-biotin tertiary antibody, it is not commonly done. Because streptavidin binds to biotin with a very high affinity, it is very commonly used to provide amplification in biotin systems in IHC or ELISA. For example, you could use streptavidin-HRP (ab64269) to detect the biotinylated secondary antibody. When using biotinylated secondary antibodies in WB, it is important to block with BSA instead of milk. Milk contains endogenous biotins that can cause very high background.

To increase the WB signal, it is more common to use a more sensitive ECL reagent or a longer exposure. Alternatively, you could try things like enriching your samples by subcellular fractionation(ab109719), loading more protein, using more primary antibody, or incubating overnight in the primary antibody. Some researchers also produce a slightly amplified signal by using a tertiary antibody. For example, you could use an unconjugated rabbit anti-mouse (ab97042)followed by an HRP conjugated anti-rabbit (ab97051).

I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for your inquiry. ab64257 is indeed suitable for the detection of Mouse and Rabbit IgG. It can be used as a secondary antibody when used with rabbit or mouse primary antibodies. About ab64256 and ab97044, all of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Please do not hesitate to contact us again if you need further assistance.

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Answer

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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