Key features and details
- Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Product nameRabbit Anti-Mouse IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies
DescriptionRabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
Human, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
Our Abpromise guarantee covers the use of ab98784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/5000.|
|ICC/IF||1/200 - 1/1000.|
|ELISA||1/20000 - 1/400000. as primary|
|WB||1/20000 - 1/400000. chemiluminescent detection|
Immunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.
Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98784 has not yet been referenced specifically in any publications.