Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (ab98784)

Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Description

    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
  • Host species

    Rabbit
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Human, Rat more details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. as primary
WB 1/20000 - 1/400000. chemiluminescent detection

Images

  • Immunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.

    Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.

    See Abreview

References

ab98784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

Since the no primary control is showing staining, it's probably an issue with the secondary antibody. But I'll be happy to send you ab53444 as a free of charge replacement for the CD68 antibody. You'll have to use an anti-rat secondary antibody with ab53444 and I strongly encourage you to use one that is pre-adsorbed against mouse so you don't run into a similar problem.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab53444.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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