For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you very much for your reply.
I did vary goat anti-mouse IgG concentration, 1:25, 1:50, 1:100. In
all cases, even without primary antibody, I do see signals in
controls. Here are the conditions used:
goat anti-mouse IgG: various conc
Primary: Mouse anti-CD68 [ED1] (Abcam ab31630) (or isotype or none)
Secondary: Rabbit anti-mouse IgG-biotin (Abcam ab98784)
HRP: Streptavidin-peroxidase polymer, ultrasensitive (Sigma S2438)
I am not 100% aware of what my supervisor has told you (as I joined
the team in the middle of this project and inherited the project from
a student. I myself have not tried FA-11 from Serotec in our tissue
of interest, kidney, but it did work for lung when I tried. Also, in
general, Abcam antibodies are of higher quality. So I have high hope
that FA-11 from Abcam can detect macrophage infiltration.
Asked on Oct 23 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
Since the no primary control is showing staining, it's probably an issue with the secondary antibody. But I'll be happy to send you ab53444 as a free of charge replacement for the CD68 antibody. You'll have to use an anti-rat secondary antibody with ab53444 and I strongly encourage you to use one that is pre-adsorbed against mouse so you don't run into a similar problem.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab53444.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Oct 23 2012