Rabbit Anti-Mouse IgG H&L (FITC) preadsorbed (ab7071)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameRabbit Anti-Mouse IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host speciesRabbit
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Target speciesMouse
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Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
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Minimal
cross-reactivity
Human more details -
Immunogen
Full length native Mouse IgG (purified).
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ConjugationFITC. Ex: 493nm, Em: 528nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
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ClonalityPolyclonal
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Research areas
Applications
Our Abpromise guarantee covers the use of ab7071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| IHC-P | ||
| IHC-Fr | ||
| ELISA |
Application notes
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Datasheets and documents
References
This product has been referenced in:
- Bock F et al. Activated protein C ameliorates diabetic nephropathy by epigenetically inhibiting the redox enzyme p66Shc. Proc Natl Acad Sci U S A 110:648-53 (2013). Read more (PubMed: 23267072) »
- Blakeslee D & Baines MG Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J Immunol Methods 13:305-20 (1976). Read more (PubMed: 13125) »
