Rabbit Anti-Mouse IgG H&L (FITC) preadsorbed (ab7071)

Submit a review Submit a question References (2)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG

  • Clonality

    Polyclonal

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7071 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Immunomicroscopy
    Flow Cyt
    IHC-P
    IHC-Fr
    ELISA
    Notes
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab7071? Please let us know so that we can cite the reference in this datasheet.

    ab7071 has been referenced in 2 publications.

    • Bock F  et al. Activated protein C ameliorates diabetic nephropathy by epigenetically inhibiting the redox enzyme p66Shc. Proc Natl Acad Sci U S A 110:648-53 (2013). PubMed: 23267072
    • Blakeslee D & Baines MG Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J Immunol Methods 13:305-20 (1976). PubMed: 13125

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab7071.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com