Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • Host species
    Rabbit
  • Target species
    Mouse
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/2000 - 1/10000. PubMed: 17200442

Images

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    Ab6728 was used at dilution 1/200 with the primary antibody ab81785 in IHC-P. See the review on ab81785.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    Ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.
  • ELISA - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    ELISA - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
    Ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

References

This product has been referenced in:
  • Zhang C  et al. Expression of DACT1 in children with asthma and its regulation mechanism. Exp Ther Med 15:2674-2680 (2018). Read more (PubMed: 29456669) »
  • Hong Y  et al. In vitro study on the role of SOX9 in trastuzumab resistance of adenocarcinoma of the esophagogastric junction. Exp Ther Med 15:3103-3107 (2018). Read more (PubMed: 29456713) »

See all 172 Publications for this product

Publishing research using ab6728? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

36 reviews or Q&A

10
Votes: Highest First

Immunoblot detection of B-3-Tubulin

 Excellent
Abreviews
Application
Western blot
20 μg of micro tubular protein from SH-SY5y cells were used to detect B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728 - 1: 2000 dilution for 1h).
abreview image
Username

Abcam user community

Verified customer

Submitted Feb 28 2018

Excellent 2nd antibody for WB

 Excellent
Abreviews
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)
abreview image
Username

Mr. Baptiste Jude

Verified customer

Submitted Oct 10 2016

This is a good 2nd antibody

 Excellent
Abreviews
Application
Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.
abreview image
Username

Abcam user community

Verified customer

Submitted May 30 2014

The product is good

 Excellent
Abreviews
Application
Western blot
When I perform WB, that is very sensitive. making band is clearly
Username

Abcam user community

Verified customer

Submitted Jan 23 2014

good for mouse primary antibody

 Excellent
Abreviews
Application
Western blot
strong detection
abreview image
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Our customer purchased ab6728 when we had promotion last December and opened and used recently. It showed high background so the customer tried several times but failed to optimize the condition.
So, the customer tried other brand’s(GenDEPOT)...

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Thank you for your email. I am sorry to hear that customer have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further suggestions for troubleshooting;

- Please try 5...

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ecd a problem with our product, which including mouse anti His Tag (4D11) cat no. AB5000 (lot GR44936-1) and AB6728 (Lot GR15565-6) and wants to claim this item under the abpromise guarantee. Her detailed information about her condition using to detect...

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Thank you for contacting us. I am sorry to hear these antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I apprec...

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Dear Technical Team,

Hope this e-mail finds you well!

Kindly suggest the exact concentration of Rabbit polyclonal Secondary Antibody to Mouse IgG - H+L (HRP) (ab6728) since on it's datasheet the concentration is mentioned as 1 mg ...

Read More

Thank you for your message.

I am pleased to help answer your question.

I can confirm that '1 mg at 2mg/ml' means that the vial contains 1 mg of antibody at a concentration of 2 mg.ml. The concentration is 2 mg/ml. (Therefore the v...

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I'm confused because when I had inquiry about the precipitation before, I received emailusing them with gentle vortex but,
this time, you answered spin down it and use only supernatant.
Let me know which method is right.


Best...

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Thank you for your reply.

I can confirm that for the HRP conjugated antibodies the approach that Irecommended is the correct one. Spining the vial down will prevent free HRP to bind to un-specifically to samples and therefore avoid increased ...

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Dear technical team,

We usually recommendab6721, ab6741, ab6789 and ab6728 secondary antibodies because those have many references,
and many customers use them now.
But, there's some precipitation, sometimes we have complain about ...

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Thank you for your enquiry. I am sorry to hear the customer has concerns regarding the quality of ab6721, ab6741, ab6789 and ab6728.

As the antibody does contain proteins and other solutes it would not be abnormal to see a little cloudiness ...

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1-10 of 36 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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