Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

Thank you for contacting us and for letting us know about this precipitate. I'm sorry that you did not receive the reply sent initially to your complaint (Monday 7th November, 03:49 GMT), reply sent Monday 7th of November, 17:23. I have summarised the original response below: It is not uncommon for a small amount of brown precipitate to be formed with HRP conjugated antibodies such as the one you have purchased. This does not usually affect the activity of the antibody.  We would  encourage you to try this antibody and let us know if there are any problems with the results. However, if you still have concerns and do not wish to try this vial, I will be happy to send a replacement vial of ab6728 or a different anti-mouse secondary antibody. Please keep me updated of the results with this vial, or let me know if you would prefer the replacement. I apologize for any inconvenience this issue has caused.

Thank you for contacting us. I am sorry to hear you have not been satisfied with the products provided to you by us. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Thank you for performing the experiment to check the purity of the antibody. I would be very interested to see those images if you would not mind sharing them with me? The information provided to you by our distributes in Taiwan is correct. This kind of precipitate is relatively frequently formed with HRP conjugated antibodies. I am currently looking into exactly why this happens and how we can prevent it from happening in the future, however, the precipitate does not usually affect the performance of the antibody. May I ask, have you tried using the antibodies in your experiments? If not I would suggest this is a good idea as there is usually no problem with the activity of the antibodies. If you find that they are not performing as you would expect we would of course replace your antibody if it had been used as stated advised the datasheet. Again, I am sorry that you have been dissatisfied with the products purchased. I hope you find that they work as expected, otherwise please do let me know.

Thank you for your enquiry. Please find attached the CoCs you requested. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for contacting us and for letting us know about this precipitate. The antibody ab6728 does often contain a brown precipitate, and it should perform as expected. We encourage you to try this antibody and let us know if there are any problems with the results. However, if you still have concerns and do not wish to try this vial, I will be happy to send a replacement vial of ab6728 or a different anti-mouse secondary antibody. Please keep me updated of the results with this vial, or let me know if you would prefer the replacement. I apologize for any inconvenience this issue has caused.

I have looked into your report of precipitation of the secondary HRP antibody you have received, ab6728. I am pleased to inform you that this is normal with this antibody due to the HRP conjugation and should not affect the activity of the antibody. I would recommend gently vortexing and using as normal. However, if you find that the antibody does not perform as expected please let us know and we will be happy to offer a free of charge replacement or refund under our Abpromise guarantee. More information of this can be found here: https://www.abcam.com/index.html?pageconfig=resource&rid=10372 If you have any further questions please do not hesitate to contact us again.

Thank you very much for informing us about this problem of the customer. Indeed, this HRP conjugated antibody is known for potentially having precipitates inside. This is due to the HRP conjugation and won't affect the performance of the antibody. We had recently followed up with customers who had this precipitate and tried the antibody. They told us that the antibody was working well. I do suggest therefore, if the clot the customer is seeing is brownish and looks like a precipitate, than the customer can try this antibody as usual. If there is nevertheless a problem with the results, please advise us and we will immediately replace the product. Thank you very much for your help. Please let me know if you have any questions.

Thank you for contacting us. Many of our customers have reported similar issues to us. We are completely aware of this problem. We have followed the complaints to many of our customers; their feedback says the antibody worked great, they found no problem at all. Please be advised that the brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. If in case the antibody fails we will be happy to offer a free of charge replacement under our Abpromise guarantee. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. The results that you have been obtaining are indeed concerning. You are detecting two bands most likely human heavy and light IgG. Please can you tell me whether these bands are of an appropriate size for this conclusion or whether the reactivity could be attributable to something else. I look forward to hearing from you.

Thank you for your enquiry. The antigen retrieval method for this antibody is heat-induced epitope retrieval at a high pH (EDTA, pH 8-9). You may find a detailed protocol at IHCWorld.com: http://www.ihcworld.com/_protocols/epitope_retrieval/tris_edta.htm Unfortunately I do not have an image of this antibody used in Western blotting or IHC. I do not see that the antibody was tested in brain tissue, but human liver showed positive staining along luminal surfaces of bile canaliculi, and human colon showed positive staining on the luminal surface of secretory epithelium. In Western blot, the band size is predicted to be 141kDa, but may run slightly higher due to glycosylation. I do not know how many freeze/thaw cycles it would take to severely damage the antibody. It is recommended to aliquot this antibody before freezing. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Mouse IgG antibody (ab6728) has been purified by immunoaffinity chromatography. It has not been preabsorbed. You may wish to take a look at the numerous favourable reviews that we have received about this antibody. You can examine these by clicking on the "Abreviews" tab on the antibody datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.