Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
- Datasheet
- References (306)
- Protocols
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (HRP)
See all IgG secondary antibodies -
Description
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details -
Immunogen
Mouse IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance. -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab6728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | 1/1000. | |
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| ICC/IF | 1/1000 - 1/5000. | |
| WB | 1/2000 - 1/10000. PubMed: 17200442 |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.
Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).
Black arrows indicate double-positive cells.
Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.
Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) -
Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)This image is courtesy of an anonymous Abreview.ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.
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ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
References
This product has been referenced in:
- Jiang L et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). Read more (PubMed: 31232476) »
- Tian BY et al. Specific knockdown of WNT8b expression protects against phosphate-induced calcification in vascular smooth muscle cells by inhibiting the Wnt-ß-catenin signaling pathway. J Cell Physiol 234:3469-3477 (2019). Read more (PubMed: 30461014) »
