Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • Host species
    Rabbit
  • Target species
    Mouse
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/2000 - 1/10000. PubMed: 17200442

Images

  • Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.

    Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

    Black arrows indicate double-positive cells.

    Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

    Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

  • ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.

  • ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • ab6728 was used at dilution 1/200 with the primary antibody ab81785 in IHC-P. See the review on ab81785.

  • ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

References

This product has been referenced in:
  • Zhang C  et al. Expression of DACT1 in children with asthma and its regulation mechanism. Exp Ther Med 15:2674-2680 (2018). Read more (PubMed: 29456669) »
  • Hong Y  et al. In vitro study on the role of SOX9 in trastuzumab resistance of adenocarcinoma of the esophagogastric junction. Exp Ther Med 15:3103-3107 (2018). Read more (PubMed: 29456713) »
See all 217 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Western blot
The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).

Figure legend
Western blot image of actin bands. HCT116 cells transfected with non-targeting siRNA (lane #1) or CDK4/6 siRNA (lane #2).

Abcam user community

Verified customer

Submitted Oct 02 2018

Abreviews
Application
Western blot
20 μg of micro tubular protein from SH-SY5y cells were used to detect B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728 - 1: 2000 dilution for 1h).

Abcam user community

Verified customer

Submitted Feb 28 2018

Abreviews
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)

Mr. Baptiste Jude

Verified customer

Submitted Oct 10 2016

Abreviews
Application
Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.

Abcam user community

Verified customer

Submitted May 30 2014

Abreviews
Application
Western blot
When I perform WB, that is very sensitive. making band is clearly

Abcam user community

Verified customer

Submitted Jan 23 2014

Abreviews
Application
Western blot
strong detection

Abcam user community

Verified customer

Submitted Jul 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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