Application
Western blot
loaded 20ug human cell lysate
reduced and denatured conditions
primary antibody was a mouse-anti-human & incubated for 16hr at 4degrees in 5% milk
secondary (ab6728) incubated for 1hr, at 1:5000 in 5% milk at 20degrees
from left to right:
Lane 1 = human cell lysate
Lane 2 = human cell lysate
Ladder
reduced and denatured conditions
primary antibody was a mouse-anti-human & incubated for 16hr at 4degrees in 5% milk
secondary (ab6728) incubated for 1hr, at 1:5000 in 5% milk at 20degrees
from left to right:
Lane 1 = human cell lysate
Lane 2 = human cell lysate
Ladder
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Submitted Mar 31 2022