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  1. Link

    rabbit-mouse-igg-hl-tritc-ab6725.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Mouse IgG H&L (TRITC) (ab6725)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit Anti-Mouse IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF

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Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Rabbit IgG
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • TRITC

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
ICC/IF
1/1000 - 1/5000. PubMed: 19362545
Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
ICC/IF
1/1000 - 1/5000. PubMed: 19362545

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab6725? Please let us know so that we can cite the reference in this datasheet.

ab6725 has been referenced in 5 publications.

  • Wang NK  et al. Mouse Models of Achromatopsia in Addressing Temporal "Point of No Return" in Gene-Therapy. Int J Mol Sci 22:N/A (2021). PubMed: 34360834
  • Xie P  et al. Therapeutic effect of transplantation of human bone marrow-derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion. Mol Med Rep 20:3065-3074 (2019). PubMed: 31432152
  • Qiao J  et al. SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner. Oncotarget 7:46088-46099 (2016). IHC-P . PubMed: 27323859
  • Zavala-Zendejas VE  et al. Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. Cancer Invest 29:1-11 (2011). IF . PubMed: 20874001
  • Koh S  et al. STIM1 regulates store-operated Ca2+ entry in oocytes. Dev Biol 330:368-76 (2009). ICC/IF . PubMed: 19362545

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Hola
Recientemente hemos comprado tres anticuerpos para immunofluorescencia y uno de ellos no funciona bien (te adjunto imagenes)
Da mucho background y hay unos “pegotes” que no hay manera de eliminar ni diluyendo el anticuerpo ni bloqueando con suero ni alargando los lavados.
Me gustaria saber como podemos arreglar el tema.
El anticuerpo es el ab6725 Rabbit Polyclonal Secondary Antibody to Mouse igG-TRITC
Dime lo que podemos hacer
Saludos y gracias!!

Read More

Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Muchas gracias por contactarnos y alertarnos del problema experimentado con este anticuerpo.
Lamento mucho que no os esté funcionando como debería. Todos los productos de Abcam tienen seis meses de garantía desde la fecha de compra, y si resultara que el anticuerpo ha salido efectivamente defectuoso, en ese caso os mandaríamos un reemplazo gratuito, o bien un reembolso o vale sin caducidad.
Antes de concluir que el problema resida en la calidad del secundario, me gustaría consultarte algunas dudas:
- Respecto al anticuerpo primario usado con ab6725, ¿Qué isotipo tiene? ¿De qué especie procede? ¿Está validado para usar en ICC? ¿Habéis usado este primario satisfactoriamente con otro secundario?
- Y el secundario, ¿habéis probado a utilizarlo con otros primarios?
- Respecto al protocolo seguido y las muestras usadas, ¿son las mismas que se han utilizado con éxito usando los otros anticuerpos que mencionas?
- ¿Has comprobado la tinción que se observa al incubar el secundario sin anticuerpo primario?
- Te agradecería que por favor me facilitaras el número de lote y de pedido del anticuerpo, para descartar que haya podido haber cualquier problema durante el envío.
Muchas gracias por tu colaboración, y quedo a la espera de tu respuesta.

Read More

Abcam Scientific Support

Answered on Jun 28 2012

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