Overview

  • Product name
    Rabbit Anti-Mouse IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Mouse
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000. PubMed: 19362545

References

This product has been referenced in:
  • Qiao J  et al. SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner. Oncotarget 7:46088-46099 (2016). IHC-P . Read more (PubMed: 27323859) »
  • Zavala-Zendejas VE  et al. Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. Cancer Invest 29:1-11 (2011). IF . Read more (PubMed: 20874001) »

See all 3 Publications for this product

Publishing research using ab6725? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

Hola
Recientemente hemos comprado tres anticuerpos para immunofluorescencia y uno de ellos no funciona bien (te adjunto imagenes)
Da mucho background y hay unos “pegotes” que no hay manera de eliminar ni diluyendo el anticuerpo ni...

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Muchas gracias por contactarnos y alertarnos del problema experimentado con este anticuerpo.
Lamento mucho que no os esté funcionando como debería. Todos los productos de Abcam tienen seis meses de garantía desde la fecha de compra, y si resultara...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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