Rabbit Anti-Mouse IgG H&L (TRITC) (ab6725)
Key features and details
- Rabbit Anti-Mouse IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (TRITC)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details -
Immunogen
Mouse IgG whole molecule
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
1/10000 - 1/50000.
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| ICC/IF |
1/1000 - 1/5000. PubMed: 19362545
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| Notes |
|---|
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Immunomicroscopy
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
1/10000 - 1/50000. |
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ICC/IF
1/1000 - 1/5000. PubMed: 19362545 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6725 has been referenced in 4 publications.
- Xie P et al. Therapeutic effect of transplantation of human bone marrow-derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion. Mol Med Rep 20:3065-3074 (2019). PubMed: 31432152
- Qiao J et al. SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner. Oncotarget 7:46088-46099 (2016). IHC-P . PubMed: 27323859
- Zavala-Zendejas VE et al. Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. Cancer Invest 29:1-11 (2011). IF . PubMed: 20874001
- Koh S et al. STIM1 regulates store-operated Ca2+ entry in oocytes. Dev Biol 330:368-76 (2009). ICC/IF . PubMed: 19362545