Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

Submit a review Q&A (1)References (7)

Overview

  • Product name

    Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
    See all IgG + IgM + IgA secondary antibodies

  • Host species

    Rabbit

  • Target species

    Mouse

  • Specificity

    Antibodies were raised in rabbit to mouse IgG, IgM and IgA

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.

  • Conjugation notes

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.

  • Clonality

    Polyclonal

  • General notes

    F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 15483223
IHC-P Use at an assay dependent dilution.

Images

  • Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

References

This product has been referenced in:

  • Ahn J  et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . Read more (PubMed: 26432097) »
  • Michalski A  et al. Surveillance of hantaviruses in Poland: a study of animal reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis 14:514-22 (2014). Read more (PubMed: 24902039) »
See all 7 Publications for this product

Publishing research using ab8517? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

Read More
Answer

When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com