Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

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Overview

  • Product name
    Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
    See all IgG + IgM + IgA secondary antibodies
  • Host species
    Rabbit
  • Target species
    Mouse
  • Specificity
    Antibodies were raised in rabbit to mouse IgG, IgM and IgA
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • General notes
    F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 15483223
IHC-P Use at an assay dependent dilution.

Images

  • Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
    Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
    Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

References

This product has been referenced in:
  • Ahn J  et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . Read more (PubMed: 26432097) »
  • Michalski A  et al. Surveillance of hantaviruses in Poland: a study of animal reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis 14:514-22 (2014). Read more (PubMed: 24902039) »
See all 7 Publications for this product

Publishing research using ab8517? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

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Answer

When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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