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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (10)

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Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

    Key features and details

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: ICC/IF, IHC-P

    You may also be interested in

    Blocking
    Normal Rabbit Serum (ab166640)

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    Overview

    • Product name

      Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
      See all IgG + IgM + IgA secondary antibodies
    • Host species

      Rabbit
    • Target species

      Mouse
    • Specificity

      Antibodies were raised in rabbit to mouse IgG, IgM and IgA
    • Tested applications

      Suitable for: ICC/IF, IHC-Pmore details
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.50
      Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
    • Conjugation notes

      The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
    • Clonality

      Polyclonal
    • General notes

      F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgA
      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Secondary antibodies
      • anti-Mouse
      • IgG + IgM + IgA
      • Fluorophore
      • FITC

    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab8517 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      ICC/IF
      Use at an assay dependent dilution. PubMed: 15483223
      IHC-P
      Use at an assay dependent dilution.
      Notes
      ICC/IF
      Use at an assay dependent dilution. PubMed: 15483223
      IHC-P
      Use at an assay dependent dilution.

      Images

      • Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
        Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
        Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (10)

      Publishing research using ab8517? Please let us know so that we can cite the reference in this datasheet.

      ab8517 has been referenced in 10 publications.

      • Zhao M  et al. The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells. Cell Prolif 53:e12758 (2020). PubMed: 31922317
      • Akyash F  et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod Biomed (Yazd) 17:891-906 (2019). PubMed: 31970311
      • Kolodziej M  et al. First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland. Ann Agric Environ Med 25:368-373 (2018). PubMed: 29936795
      • Ahn J  et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . PubMed: 26432097
      • Michalski A  et al. Surveillance of hantaviruses in Poland: a study of animal reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis 14:514-22 (2014). PubMed: 24902039
      • Guo Z  et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739
      • Tang L  et al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 226:832-42 (2011). IHC-P . PubMed: 20857425
      • Huang CY  et al. Plasticity of stem cells derived from adult periodontal ligament. Regen Med 4:809-21 (2009). PubMed: 19903001
      • Cai L  et al. Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy. J Am Coll Cardiol 48:1688-97 (2006). PubMed: 17045908
      • Pfister-Genskow M  et al. Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer: improper reprogramming of genes required for development. Biol Reprod 72:546-55 (2005). ICC/IF ; Mouse . PubMed: 15483223

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 15 2010

      Answer

      When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM.

      Read More

      Abcam Scientific Support

      Answered on Jun 15 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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