• Product name

    Rabbit Anti-Pig IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Host species

  • Target species

  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    pig IgG whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality

  • Research areas


Our Abpromise guarantee covers the use of ab6775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References

    This product has been referenced in:

    • Freitas FB  et al. The QP509L and Q706L superfamily II RNA helicases of African swine fever virus are required for viral replication, having non-redundant activities. Emerg Microbes Infect 8:291-302 (2019). Read more (PubMed: 30866783) »
    • Freitas FB  et al. African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycle. Sci Rep 8:3471 (2018). Read more (PubMed: 29472632) »
    See all 4 Publications for this product

    Customer reviews and Q&As


    Thank you very much for your inquiry and your interest in our products.

    If performing a double or triple staining, it is very important to make all the controls that the secondary antibodies do not crossreact with the immunoglobulins from the other species. For example for the anti pig antibody, it would be important that the anti pig antibody does not recognise the immunoglobulins from mouse or rabbit, not to have a false collocatisation. Unfortunately, non of our anti pig antibodies have been tested for this. However as you have used the ab6775 already successfully in your laboratory if I understood correctly, I can recommend to use the ab6773 as it is from the same original pool. Please do however perform the single color controls to verify the crossreactivity of the ab6773 with rabbit or mouse immunoglobulins.

    In regards to the anti rabbit antibody, I can recommend to use the ab98500, a donkey anti rabbit IgG which has been preabsorbed against mouse, rat and human immunoglobulins, sothere is nocrossreaction against those immunoglobulins. We do not have an anti rabbit DyLight 594 which has been crossabsorbed also against the pig immunoglobulins. If you are concerned or if you have troubles with background staining on the VERO cells, I can recommend to use the same antibody , however with no Fc region, i.e. the F(ab')2 portion. This would be the ab98490.
    https://www.abcam.com/index.html?datasheet=98500 (or use the following: https://www.abcam.com/index.html?datasheet=98500).
    https://www.abcam.com/index.html?datasheet=98490 (or use the following: https://www.abcam.com/index.html?datasheet=98490).
    Please let us know which one you would prefer, so we can send the correctproforma invoice.

    The species in which the secondary antibody is generated is not very important. It is only important if you get background staining from the secondary antibody alone, in which case you can then block with serum of that species. However this is not an issue in general, especially not in cell culture.

    I hope this information is helpful.

    We will send theproforma invoices tomorrow also to your email address. I hope this is how you would like to receive it. Please let me know however whether you would like us to send it to another email address or fax.

    Thank you very much. Please do not hesitate to contact us for any further question or advice.

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