Product nameRabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
Rat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
General notesPart of the AbExcel range.
Our Abpromise guarantee covers the use of ab6735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/5000 - 1/50000.|
|ELISA||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
ab6735 has not yet been referenced specifically in any publications.