Overview

  • Product name

    Rabbit Anti-Rat IgG H&L (beta-galactosidase)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WB, ELISA, ICCmore details
  • Immunogen

    Highly purified whole immunoglobulin from Rat.

  • Conjugation

    beta-galactosidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136716 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/100 - 1/1000.
ICC Use at an assay dependent concentration.

References

ab136716 has not yet been referenced specifically in any publications.

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