Key features and details
- Rabbit Anti-Rat IgG H&L (beta-galactosidase)
- Conjugation: beta-galactosidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, WB, ELISA, ICC
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136716 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
General notesPreparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.
Our Abpromise guarantee covers the use of ab136716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ELISA||1/100 - 1/1000.|
|ICC||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab136716 has not yet been referenced specifically in any publications.