Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

Submit a review Q&A (3)References (11)

Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
  • Host species
    Rabbit
  • Target species
    Rat
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA 1/500000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)
    Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
    Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)
    Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)
    Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

References

This product has been referenced in:
  • Xiao R & Chen R Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury. Mol Med Rep 15:3535-3540 (2017). Read more (PubMed: 28393227) »
  • Dinh CH  et al. Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice. J Histochem Cytochem 64:237-55 (2016). Read more (PubMed: 26920068) »
See all 11 Publications for this product

Publishing research using ab6733? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

background with ab6733 on Mouse tissue samples

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Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (https://www.abcam.com/ab7103), le numéro de commande de remplacement gratuit de ce produit est *****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

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Question

Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

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Answer

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Question

Is this or any other anti-rat Ab absorbed to prevent cross reactivity with mouse Abs ? I need to run a double label withj mouse and rat Abs and I need to prevent any cross reactivity. I can't use any rabbit Abs for another technical reason. Thanks David

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Answer

I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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