Rabbit Anti-Rat IgG H&L (Biotin) preadsorbed (ab7103)

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Overview

  • Product name

    Rabbit Anti-Rat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Rat

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/20000 - 1/60000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab7103 has not yet been referenced specifically in any publications.

    Publishing research using ab7103? Please let us know so that we can cite the reference in this datasheet.

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