Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Rat
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Images

  • Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

References

This product has been referenced in:
  • Kneppers A  et al. A novel in vitro model for the assessment of postnatal myonuclear accretion. Skelet Muscle 8:4 (2018). Read more (PubMed: 29444710) »
  • Gholinejad M  et al. Adenosine decreases oxidative stress and protects H2O2-treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8:439-446 (2018). Read more (PubMed: 29732147) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Secondary ab

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
It works good with abcam as well as other vendors antibodies

Abcam user community

Verified customer

Submitted Sep 05 2014

Answer

Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view Cy5 might be brighter than FITC and an amplification with HRP or biotin can also enhance greatly your signal if you suspect low abundance of the protein in your samples). You have not mentioned the source of your cells, but please remember the antibody is a rat monoclonal so it cannot be used on rat cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More

Answer

We have had no feedback from customers that may have used these antibodies in plant.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up