Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
Immunomicroscopy
Flow Cyt
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab7100 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you very much for your reply.

    I've looked at the package'stracking information, and it arrived on September 20 at 9:34 EST. You can track the package on FedEx.com with tracking number: ***. It was signed for by ***- does that help you track it down?

    Please let me know if you can find thepackage with this information, or if you need any furtherassistance and I'll be happy to help.

    Read More

    Answer

    Thank you very much for your reply.

    It does sound like the secondary is reacting with the IgG's in the mouse serum. I'm sending a free of charge vial of ab6115 on the order *** which should arrive tomorrow.

    Please keep me updated about the results using this secondary, and if there is anything else that we can do for you please let me know.

    Read More

    Answer

    Thank you very much for contacting us with your question.

    It is possible that the secondary antibody is causing some background signal, as ab7100 has been pre-adsorbed only against human serum proteins and not mouse serum proteins. Have you determined that the secondary antibody is causing the background due to signal in a "no primary" or isotype control sample? What dilution of ab7100 are you using, and what kind of mouse cells are you analyzing? What kind of blocking solution do you use?

    If you know that the background is coming from the secondary antibody, I'd suggest using a secondary antibody that has been pre-adsorbed against mouse serum proteins. Ab6115 is a goat anti-rat antibody that has been pre-adsorbed against mouse. I'd be happy to send this as a replacement for ab7100 if it is not working as it should be. Please send me your order or PO number if you would like to receive this replacement.

    I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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