Overview

  • Product name
    Rabbit Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-P, ICC, ELISA, WBmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/5000.
ICC 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (as primary)
WB 1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).

Images

  • Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)
    Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)

    All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/2000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/10000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

References

ab102172 has not yet been referenced specifically in any publications.

Publishing research using ab102172? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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