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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Rat IgG H&L (HRP) (ab102172)

  • Datasheet
  • SDS
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Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172)

    Key features and details

    • Rabbit Anti-Rat IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: IHC-P, ICC, ELISA, WB

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    Overview

    • Product name

      Rabbit Anti-Rat IgG H&L (HRP)
      See all IgG secondary antibodies
    • Host species

      Rabbit
    • Target species

      Rat
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
    • Tested applications

      Suitable for: IHC-P, ICC, ELISA, WBmore details
    • Conjugation

      HRP

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      Part of the AbExcel range.
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Enzyme
      • HRP

    Associated products

    • Substrate reagent

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

    Applications

    Our Abpromise guarantee covers the use of ab102172 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/200 - 1/5000.
    ICC 1/200 - 1/5000.
    ELISA 1/10000 - 1/100000. (as primary)
    WB 1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).

    Images

    • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172)
      Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172)
      Lane 1 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
      Lanes 2-6 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

      All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/2000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
      Lanes 3-4 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/10000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
      Lane 5 : Goat polyclonal to RUNX2 (ab102712) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
      Lane 6 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab102172? Please let us know so that we can cite the reference in this datasheet.

    ab102172 has been referenced in 3 publications.

    • Bochnakian A  et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
    • Fung L  et al. miR-128 Restriction of LINE-1 (L1) Retrotransposition Is Dependent on Targeting hnRNPA1 mRNA. Int J Mol Sci 20:N/A (2019). PubMed: 31010097
    • Idica A  et al. MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1. J Biol Chem 292:20494-20508 (2017). PubMed: 28974576

    Customer reviews and Q&As

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