Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
Key features and details
- Rabbit Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
Overview
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Product name
Rabbit Anti-Rat IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Tested applications
Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
Immunogen
Rat IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/5000.
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| ELISA |
1/60000.
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|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/2500.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/5000. |
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ELISA
1/60000. |
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Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/2500. |
|
IHC-Fr
Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.
ab25377 staining Ly6g in mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS/1% BSA) for 12 hours at 4°C. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/200) was used as the secondary antibody.
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Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734) -
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (52)
ab6734 has been referenced in 52 publications.
- Zhu N et al. Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1a/BNIP3 Pathway. Front Pharmacol 11:367 (2020). PubMed: 32292345
- Patrussi L et al. p66Shc deficiency in the Eµ-TCL1 mouse model of chronic lymphocytic leukemia enhances leukemogenesis by altering the chemokine receptor landscape. Haematologica N/A:N/A (2019). PubMed: 30819907
- Li EY et al. LncRNA MIAT overexpression reduced neuron apoptosis in a neonatal rat model of hypoxic-ischemic injury through miR-211/GDNF. Cell Cycle 18:156-166 (2019). PubMed: 30563429
- Ding Y et al. MicroRNA-93 inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting the TLR4/NF-?B signaling pathway. Int J Mol Med 43:779-790 (2019). PubMed: 30569118
- Li N et al. LPS-induced CXCR7 expression promotes gastric Cancer proliferation and migration via the TLR4/MD-2 pathway. Diagn Pathol 14:3 (2019). PubMed: 30636642