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    rabbit-rat-igg-hl-hrp-ab6734.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (52)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

Key features and details

  • Rabbit Anti-Rat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr

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Overview

  • Product name

    Rabbit Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Rat
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Enzyme
    • HRP

Associated products

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/5000.
ELISA
1/60000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-Fr
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/60000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-Fr
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.

    ab25377 staining Ly6g in mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS/1% BSA) for 12 hours at 4°C. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/200) was used as the secondary antibody.

    See Abreview

  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
    Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
    Ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.

    ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

    IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (52)

Publishing research using ab6734? Please let us know so that we can cite the reference in this datasheet.

ab6734 has been referenced in 52 publications.

  • Zhu N  et al. Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1a/BNIP3 Pathway. Front Pharmacol 11:367 (2020). PubMed: 32292345
  • Patrussi L  et al. p66Shc deficiency in the Eµ-TCL1 mouse model of chronic lymphocytic leukemia enhances leukemogenesis by altering the chemokine receptor landscape. Haematologica N/A:N/A (2019). PubMed: 30819907
  • Li EY  et al. LncRNA MIAT overexpression reduced neuron apoptosis in a neonatal rat model of hypoxic-ischemic injury through miR-211/GDNF. Cell Cycle 18:156-166 (2019). PubMed: 30563429
  • Ding Y  et al. MicroRNA-93 inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting the TLR4/NF-?B signaling pathway. Int J Mol Med 43:779-790 (2019). PubMed: 30569118
  • Li N  et al. LPS-induced CXCR7 expression promotes gastric Cancer proliferation and migration via the TLR4/MD-2 pathway. Diagn Pathol 14:3 (2019). PubMed: 30636642
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

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1-2 of 2 Abreviews

DAB staining for Neutrophils

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA fixed)
worked good for DAB staining in placenta for neutrophils at 1:200 dilution for 1 hrs.

Abcam user community

Verified customer

Submitted Nov 13 2013

Detection of E-cadherin in mouse ES cell whole cell lysates.

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Secondary antibody worked very well. Clear bands seen. Other marks on film are pen marks only.

Dr. Sarah Ritson

Verified customer

Submitted Oct 31 2013

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